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mitomycin c inactivated fibroblasts  (ATCC)


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    ATCC mitomycin c inactivated fibroblasts
    Mitomycin C Inactivated Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1268 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inactivated+human+foreskin+fibroblasts/HFF-1%3B+fibroblast%3B+Human+foreskin/pm39490209-46-11-14
    Average 98 stars, based on 1268 article reviews
    mitomycin c inactivated fibroblasts - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Modification:

    Article Title: Mitochondrial Fusion by M1 Promotes Embryoid Body Cardiac Differentiation of Human Pluripotent Stem Cells
    Article Snippet: .. The human iPS-Foreskin-2 cell line, kindly provided by James A. Thomson (University of Winconsin) [ ], was propagated on a feeder layer of mitotically inactivated human foreskin fibroblasts (HFF:D551; ATCC, VA, USA) in Dulbecco's modified Eagle's medium (DMEM)/F-12 GlutaMAX medium supplemented with 20% knockout serum replacement, 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 50 U/mL penicillin/streptomycin (all from Thermo Fisher Scientific, VIC, Australia), and 20 ng/mL recombinant human fibroblast growth factor-2 (Merck Millipore, CA, USA). ..

    Article Title: Trichostatin A Enhances Differentiation of Human Induced Pluripotent Stem Cells to Cardiogenic Cells for Cardiac Tissue Engineering
    Article Snippet: .. The human Friedreich's ataxia iPS cell line FA3 was also used in this study [ 11 ]. iPS(Foreskin)-1 (passages 38–74), iPS(Foreskin)-2 (passages 23–60), and FA3 (passages 16–19) were maintained on a feeder layer of mitotically inactivated human foreskin fibroblasts (HFF:D551; American Type Culture Collection, Manassas, VA, http://www.atcc.org ) in culture media containing Dulbecco's modified Eagle's medium (DMEM)/F-12 GlutaMAX medium supplemented with 20% knockout serum replacement, 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 50 U/ml penicillin/streptomycin (all from Invitrogen, Carlsbad, CA, http://www.invitrogen.com ), and 20 ng/ml recombinant human fibroblast growth factor-2 (Millipore, Billerica, MA, http://www.millipore.com ). ..

    Article Title: Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells.
    Article Snippet: .. Human iPSCs were maintained on a feeder layer of mitotically inactivated human foreskin fibroblasts (HFF:D551; ATCC, VA, USA) in Dulbecco's modified Eagle's medium (DMEM)/F-12 GlutaMAX medium supplemented with 20% knockout serum replacement, 0.1mM 2-mercaptoethanol, 0.1mM nonessential amino acids, 50 U/mL penicillin/streptomycin (all from Thermo Fisher Scientific, VIC, Australia) and 20 ng/mL recombinant human fibroblast growth factor-2 (Merck Millipore, CA, USA)35. .. For feeder-free culture, human iPSCs were maintained and propagated on vitronectincoated plate in TeSR-E8 medium (Stem Cell Technologies, VA, Canada) according to the manufacturer’s protocol.

    Article Title: In vitro electroretinogram for the study of the functionality of differentiated retinal pigment epithelium cells.
    Article Snippet: The purpose of this study is to develop and test a method to reveal if the retinal pigment epithelium (RPE) cells differentiated from human embryonic stem cells (hESC) support the functions of photoreceptors. hESCderived RPE (hESC–RPE) cells offer a potent cell source for cell replacement therapy that may be used to prevent certain eye diseases.. Methods to assure the functionality of the RPE cells are well warranted.. Electroretinograms (ERG) measure the electrophysiological response of the retina to light stimuli.

    Article Title: Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells
    Article Snippet: .. Human iPSCs were maintained on a feeder layer of mitotically inactivated human foreskin fibroblasts (HFF:D551; ATCC, VA, USA) in Dulbecco's modified Eagle's medium (DMEM)/F-12 GlutaMAX medium supplemented with 20% knockout serum replacement, 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 50 U/mL penicillin/streptomycin (all from Thermo Fisher Scientific, VIC, Australia) and 20 ng/mL recombinant human fibroblast growth factor-2 (Merck Millipore, CA, USA) . .. For feeder-free culture, human iPSCs were maintained and propagated on vitronectin-coated plate in TeSR-E8 medium (Stem Cell Technologies, VA, Canada) according to the manufacturer’s protocol.

    Knock-Out:

    Article Title: Mitochondrial Fusion by M1 Promotes Embryoid Body Cardiac Differentiation of Human Pluripotent Stem Cells
    Article Snippet: .. The human iPS-Foreskin-2 cell line, kindly provided by James A. Thomson (University of Winconsin) [ ], was propagated on a feeder layer of mitotically inactivated human foreskin fibroblasts (HFF:D551; ATCC, VA, USA) in Dulbecco's modified Eagle's medium (DMEM)/F-12 GlutaMAX medium supplemented with 20% knockout serum replacement, 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 50 U/mL penicillin/streptomycin (all from Thermo Fisher Scientific, VIC, Australia), and 20 ng/mL recombinant human fibroblast growth factor-2 (Merck Millipore, CA, USA). ..

    Article Title: Trichostatin A Enhances Differentiation of Human Induced Pluripotent Stem Cells to Cardiogenic Cells for Cardiac Tissue Engineering
    Article Snippet: .. The human Friedreich's ataxia iPS cell line FA3 was also used in this study [ 11 ]. iPS(Foreskin)-1 (passages 38–74), iPS(Foreskin)-2 (passages 23–60), and FA3 (passages 16–19) were maintained on a feeder layer of mitotically inactivated human foreskin fibroblasts (HFF:D551; American Type Culture Collection, Manassas, VA, http://www.atcc.org ) in culture media containing Dulbecco's modified Eagle's medium (DMEM)/F-12 GlutaMAX medium supplemented with 20% knockout serum replacement, 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 50 U/ml penicillin/streptomycin (all from Invitrogen, Carlsbad, CA, http://www.invitrogen.com ), and 20 ng/ml recombinant human fibroblast growth factor-2 (Millipore, Billerica, MA, http://www.millipore.com ). ..

    Article Title: Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells.
    Article Snippet: .. Human iPSCs were maintained on a feeder layer of mitotically inactivated human foreskin fibroblasts (HFF:D551; ATCC, VA, USA) in Dulbecco's modified Eagle's medium (DMEM)/F-12 GlutaMAX medium supplemented with 20% knockout serum replacement, 0.1mM 2-mercaptoethanol, 0.1mM nonessential amino acids, 50 U/mL penicillin/streptomycin (all from Thermo Fisher Scientific, VIC, Australia) and 20 ng/mL recombinant human fibroblast growth factor-2 (Merck Millipore, CA, USA)35. .. For feeder-free culture, human iPSCs were maintained and propagated on vitronectincoated plate in TeSR-E8 medium (Stem Cell Technologies, VA, Canada) according to the manufacturer’s protocol.

    Article Title: In vitro electroretinogram for the study of the functionality of differentiated retinal pigment epithelium cells.
    Article Snippet: The purpose of this study is to develop and test a method to reveal if the retinal pigment epithelium (RPE) cells differentiated from human embryonic stem cells (hESC) support the functions of photoreceptors. hESCderived RPE (hESC–RPE) cells offer a potent cell source for cell replacement therapy that may be used to prevent certain eye diseases.. Methods to assure the functionality of the RPE cells are well warranted.. Electroretinograms (ERG) measure the electrophysiological response of the retina to light stimuli.

    Article Title: Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells
    Article Snippet: .. Human iPSCs were maintained on a feeder layer of mitotically inactivated human foreskin fibroblasts (HFF:D551; ATCC, VA, USA) in Dulbecco's modified Eagle's medium (DMEM)/F-12 GlutaMAX medium supplemented with 20% knockout serum replacement, 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 50 U/mL penicillin/streptomycin (all from Thermo Fisher Scientific, VIC, Australia) and 20 ng/mL recombinant human fibroblast growth factor-2 (Merck Millipore, CA, USA) . .. For feeder-free culture, human iPSCs were maintained and propagated on vitronectin-coated plate in TeSR-E8 medium (Stem Cell Technologies, VA, Canada) according to the manufacturer’s protocol.

    Recombinant:

    Article Title: Mitochondrial Fusion by M1 Promotes Embryoid Body Cardiac Differentiation of Human Pluripotent Stem Cells
    Article Snippet: .. The human iPS-Foreskin-2 cell line, kindly provided by James A. Thomson (University of Winconsin) [ ], was propagated on a feeder layer of mitotically inactivated human foreskin fibroblasts (HFF:D551; ATCC, VA, USA) in Dulbecco's modified Eagle's medium (DMEM)/F-12 GlutaMAX medium supplemented with 20% knockout serum replacement, 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 50 U/mL penicillin/streptomycin (all from Thermo Fisher Scientific, VIC, Australia), and 20 ng/mL recombinant human fibroblast growth factor-2 (Merck Millipore, CA, USA). ..

    Article Title: Trichostatin A Enhances Differentiation of Human Induced Pluripotent Stem Cells to Cardiogenic Cells for Cardiac Tissue Engineering
    Article Snippet: .. The human Friedreich's ataxia iPS cell line FA3 was also used in this study [ 11 ]. iPS(Foreskin)-1 (passages 38–74), iPS(Foreskin)-2 (passages 23–60), and FA3 (passages 16–19) were maintained on a feeder layer of mitotically inactivated human foreskin fibroblasts (HFF:D551; American Type Culture Collection, Manassas, VA, http://www.atcc.org ) in culture media containing Dulbecco's modified Eagle's medium (DMEM)/F-12 GlutaMAX medium supplemented with 20% knockout serum replacement, 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 50 U/ml penicillin/streptomycin (all from Invitrogen, Carlsbad, CA, http://www.invitrogen.com ), and 20 ng/ml recombinant human fibroblast growth factor-2 (Millipore, Billerica, MA, http://www.millipore.com ). ..

    Article Title: Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells.
    Article Snippet: .. Human iPSCs were maintained on a feeder layer of mitotically inactivated human foreskin fibroblasts (HFF:D551; ATCC, VA, USA) in Dulbecco's modified Eagle's medium (DMEM)/F-12 GlutaMAX medium supplemented with 20% knockout serum replacement, 0.1mM 2-mercaptoethanol, 0.1mM nonessential amino acids, 50 U/mL penicillin/streptomycin (all from Thermo Fisher Scientific, VIC, Australia) and 20 ng/mL recombinant human fibroblast growth factor-2 (Merck Millipore, CA, USA)35. .. For feeder-free culture, human iPSCs were maintained and propagated on vitronectincoated plate in TeSR-E8 medium (Stem Cell Technologies, VA, Canada) according to the manufacturer’s protocol.

    Article Title: Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells
    Article Snippet: .. Human iPSCs were maintained on a feeder layer of mitotically inactivated human foreskin fibroblasts (HFF:D551; ATCC, VA, USA) in Dulbecco's modified Eagle's medium (DMEM)/F-12 GlutaMAX medium supplemented with 20% knockout serum replacement, 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 50 U/mL penicillin/streptomycin (all from Thermo Fisher Scientific, VIC, Australia) and 20 ng/mL recombinant human fibroblast growth factor-2 (Merck Millipore, CA, USA) . .. For feeder-free culture, human iPSCs were maintained and propagated on vitronectin-coated plate in TeSR-E8 medium (Stem Cell Technologies, VA, Canada) according to the manufacturer’s protocol.

    Cell Culture:

    Article Title: In vitro electroretinogram for the study of the functionality of differentiated retinal pigment epithelium cells.
    Article Snippet: The purpose of this study is to develop and test a method to reveal if the retinal pigment epithelium (RPE) cells differentiated from human embryonic stem cells (hESC) support the functions of photoreceptors. hESCderived RPE (hESC–RPE) cells offer a potent cell source for cell replacement therapy that may be used to prevent certain eye diseases.. Methods to assure the functionality of the RPE cells are well warranted.. Electroretinograms (ERG) measure the electrophysiological response of the retina to light stimuli.

    Suspension:

    Article Title: Transplanted Human Induced Pluripotent Stem Cell-Derived Neural Progenitor Cells Do Not Promote Functional Recovery of Pharmacologically Immunosuppressed Mice With Contusion Spinal Cord Injury.
    Article Snippet: .. Pluripotent stem cells were maintained on the top of the mitotically inactivated human foreskin fibroblasts (CRL2429; ATCC, Manassas, VA, USA), and neural differentiation was carried out in a suspension of cell spheres as described in detail in Puttonen et al. (40). ..



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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Cell

    Article Title: Unveiling the Role of the Most Impactful Cardiovascular Risk Locus through Haplotype Editing

    doi: 10.1016/j.cell.2018.11.014

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Inactivated human foreskin fibroblasts , EMD-Millipore , Cat# SCC057.

    Techniques: Virus, Recombinant, Saline, cDNA Synthesis, SYBR Green Assay, Reverse Transcription, DNA Purification, Electron Microscopy, Staining, Cell Adhesion Strength Assay, Control, Software